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Merck

S5059

Sigma-Aldrich

SigmaSpin 测序反应清除

post-reaction clean-up columns

别名:

sequencing clean up columns

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.52

品質等級

儲存溫度

2-8°C

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應用

SigmaSpin ® 测序反应清理已用于合成反义RNA探针。
非常适合用于去除测序反应中的染料终止子核苷酸和引物以及核酸探针标记反应中的放射性标记核苷酸、引物和荧光染料。

其他說明

SigmaSpin 反应后净化柱非常适合通量较低的应用,如清理探针标记反应或少量测序反应。这些净化柱可接受高达 100μ L的样品量。每个净化柱配有一个收集管,用于在离心过程中收集 DNA。

SigmaSpin 反应后净化柱提供了一种快速、简便、高效的方法,用于去除未掺入的染料、多余的盐和其他干扰反应成分。每个净化柱均填充预水合分子排阻树脂,用分子生物级水平衡,并在我们独特的净化柱设计中提供。该净化柱还包括一个扣盖底部密封件和一个 o 形环(包含螺旋盖),以确保树脂保持水合。SigmaSpin 已在 ABI PRISM ® 3700、3100、310 和 377 的高通量基因组中心和核心设施中进行了检测。每孔可接受高达 20μL 的样本量。
有关其他信息,请参阅 www.sigma-aldrich.com /sequencing

法律資訊

ABI PRISM is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SabreLite is a registered trademark of Pelican Products, Inc.
SigmaSpin is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Seong Hyeok Cho et al.
Biochemical and biophysical research communications, 498(3), 431-436 (2018-03-03)
The goal of this study is to determine the anti-cancer mechanism of Cordycepin in A549 Cisplatin-Resistance (CR) lung cancer cells. Cordycepin inhibited the viability of A549CR cells in a dose-dependent manner. The cell inhibition was due to induction of apoptosis
Kateřina Švehlová et al.
Nucleic acids research, 44(22), 10789-10803 (2016-10-30)
A fundamental motif in canonical nucleic acid structure is the base pair. Mutations that disrupt base pairs are typically destabilizing, but stability can often be restored by a second mutation that replaces the original base pair with an isosteric variant.
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)
Martin R Silic et al.
Genetics, 215(4), 1067-1084 (2020-06-18)
The roles of bioelectric signaling in developmental patterning remain largely unknown, although recent work has implicated bioelectric signals in cellular processes such as proliferation and migration. Here, we report a mutation in the inwardly rectifying potassium channel (kir) gene, kcnj13/kir7.1
Ray Chang et al.
Acta biomaterialia, 111, 221-231 (2020-05-23)
Gene transfection is important in biotechnology and is used to modify cells intrinsically. It can be conducted in cell suspension or after cell adhesion, where the efficiency is dependent on many factors such as the type of nanocarrier used and

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