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一般說明
转移RNA（tRNA）是通过苯酚-氯仿萃取和乙醇沉淀从baker′s酵母中分离得到。tRNA是一类大约具有80个核苷酸长度的RNA分子，具有苜蓿叶形的二级结构和三级L形结构。
應用
适合用作核酸纯化和沉淀的载体。
从bakers′酵母（酿酒酵母）中分离得到的核糖核酸用于：
从bakers′酵母（酿酒酵母）中分离得到的核糖核酸用于：
- 载体溶液中，作为对照的一部分用于逆转录酶–定量聚合酶反应（RT-qPCR）分析
- 在单标记原位杂交中，作为预杂交（4）和杂交缓冲液的组成部分
- 作为增强从少量细胞中回收RNA的载体
生化／生理作用
转移RNA（tRNA）在翻译过程中起重要作用。它负责向核糖体中添加氨基酸以形成肽链。tRNA包含一个用于mRNA碱基配对的反密码子区域和两个附着区域：用于氨基酸结合和tRNA合成酶识别。核糖体RNA能够促进tRNA沿mRNA的移动。
成分
tRNA 溶于 1 mM EDTA 的 10 mM Tris HCl (pH 7.4) 溶液中。
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Marilyn C Olson et al.
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