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描述
Polyadenylic acid
品質等級
形狀
lyophilized
分子量
700-3500 kDa
包裝
pkg of 100 mg
製造商／商標名
Roche
濃度
0.5 mg/mL (Working concentration)
技術
PCR: suitable
顏色
white
溶解度
water: soluble
吸光率
A290/260 nm 0.03-0.05
A280/260 nm 0.28-0.32
A250/260 nm 0.86-0.90
儲存溫度
2-8°C
一般說明
Poly（A）被用作DNA和RNA定量沉淀的载体。
應用
Poly（A）已可用于微滴式数字PCR（ddPCR）分析。
聚腺苷酸Poly(A)抑制 Exo1的核酸外切酶活性。还用作重悬冻干 gBlocks 的载体。
生化／生理作用
聚腺苷酸(Poly(A))尾部有3′末端，在细胞核产生。哺乳动物细胞中，约含 250 个核苷酸。Poly(A)可调控mRNA降解和翻译起始。胞质Poly(A)的延伸调控翻译过程。
品質
典型分析：
2.3μmol/mg Poly(A)（来自吸光度）相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A250/A260、A280/A260、A290/A260
2.3μmol/mg Poly(A)（来自吸光度）相当于一个单核苷酸单位。色谱均匀。
吸光度测定 A250/A260、A280/A260、A290/A260
序列
链长为 2.100 至 10.000 个核苷酸
單位定義
1个A260 单位相当于40 μg ssRNA。
外觀
冻干品，钾盐
準備報告
工作浓度：0.5 mg/ml
建议浓度为0.5 mg/ml。
工作溶液：建议浓度为0.5 mg/ml。
建议浓度为0.5 mg/ml。
工作溶液：建议浓度为0.5 mg/ml。
其他說明
仅用于生命科学研究。不可用于诊断。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Poly (A) tail length is controlled by the nuclear poly (A)-binding protein regulating the interaction between poly (A) polymerase and the cleavage and polyadenylation specificity factor
Kuhn U, et al.
The Journal of Biological Chemistry, 284(34), 22803-22814 (2009)
Yuichiro Miyaoka et al.
Scientific reports, 6, 23549-23549 (2016-04-01)
Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However, nonhomologous end-joining (NHEJ), an error-prone repair, acts concurrently, reducing the rate of high-fidelity edits. The identification of genome-editing conditions that
Poly (ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities
Cheruiyot A, et al.
DNA Repair, 35, 106-115 (2015)
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
Kengo Watanabe et al.
Nature communications, 12(1), 1353-1353 (2021-03-03)
Cells are under threat of osmotic perturbation; cell volume maintenance is critical in cerebral edema, inflammation and aging, in which prominent changes in intracellular or extracellular osmolality emerge. After osmotic stress-enforced cell swelling or shrinkage, the cells regulate intracellular osmolality
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