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Merck
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文件

P6874

Sigma-Aldrich

Anti- p53 抗体，小鼠单克隆

clone DO-1, purified from hybridoma cell culture

别名:

Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

DO-1, monoclonal

形狀

buffered aqueous solution

分子量

antigen ~53 kDa

物種活性

human

濃度

~2 mg/mL

技術

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells

同型

IgG2a

UniProt登錄號

P04637

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... TP53(7157)

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相关类别

一般說明

单克隆抗p53（小鼠IgG2a同种型）来源于小鼠骨髓瘤细胞（SP2细胞）和重组人野生型p53免疫的BALB/c小鼠脾细胞融合产生的DO-1杂交瘤 。 p53基因位于人染色体17p上。该基因编码在正常组织中表达的肿瘤抑制蛋白。

特異性

小鼠抗（p53）抗体可与人类（p53）发生特异性反应。

免疫原

重组人野生型 p53。表位位于人 p53 的 20-25 个氨基酸之间。

應用

小鼠单克隆抗p53抗体已被用于：
  • 半干转免疫印迹 （semi-dry western blotting）
  • 免疫印迹
  • 免疫荧光
  • 酶联免疫吸附测定（ELISA）
  • 免疫细胞化学
  • 免疫组织化学
  • 免疫沉淀
  • 流式细胞术
  • 染色质免疫沉淀（ChIP）分析

生化／生理作用

p53肿瘤抑制蛋白在细胞对DNA损伤和其他基因组畸变的反应中很重要。p53蛋白的升高会诱导包括p21awf1在内的多个基因的转录激活。研究表明野生型p53是一种序列特异性转录因子，可以直接与各种细胞和病毒蛋白相互作用。完整的p53功能对于维持细胞的非致瘤表型必不可少。p53在抑制癌症的发展、细胞周期调节和CAK激酶活性的调节中起着关键作用。

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书(COA)

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访问文档库

Hui Miao et al.
PLoS genetics, 15(5), e1008144-e1008144 (2019-05-16)
Long noncoding RNAs (lncRNAs) participate in various biological processes such as apoptosis. The function of lncRNAs is closely correlated with their localization within the cell. While regulatory potential of many lncRNAs has been revealed at specific subcellular location, the biological
Mikael S Lindström et al.
PloS one, 5(3), e9578-e9578 (2010-03-12)
Disruption of the nucleolus often leads to activation of the p53 tumor suppressor pathway through inhibition of MDM2 that is mediated by a limited set of ribosomal proteins including RPL11 and RPL5. The effects of ribosomal protein loss in cultured
Knockdown of hnRNPK leads to increased DNA damage after irradiation and reduces survival of tumor cells
Wiesmann N, et al.
Carcinogenesis, 38(3), 321-328 (2017)
Samantha J McDonnell et al.
Cell death discovery, 5, 132-132 (2019-09-12)
Specific molecular interactions that underpin the switch between ER stress-triggered autophagy-mediated cellular repair and cellular death by apoptosis are not characterized. This study reports the unexpected interaction elicited by ER stress between the plasma membrane (PM)-localized apoptosis effector PERP and
G Soria et al.
Oncogene, 25(20), 2829-2838 (2006-01-13)
p21(Cip1/WAF1) is a known inhibitor of the short-gap filling activity of proliferating cell nuclear antigen (PCNA) during DNA repair. In agreement, p21 degradation after UV irradiation promotes PCNA-dependent repair. Recent reports have identified ubiquitination of PCNA as a relevant feature
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