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形狀
lyophilized powder
比活性
≥60 units/mg protein
成份
Protein, 20-40% biuret
異物活動
ATPase ≤0.5%
phosphorylase a ≤1%
phosphorylase b ≤5%
儲存溫度
−20°C
一般說明
磷酸化酶激酶包含四个亚基，每个亚基含有α、β、γ以及sigma成分。该sigma成分结合4个钙分子，称为钙调蛋白，而其γ单位可用作催化亚基。
應用
来自兔肌肉的磷酸化酶激酶已应用于一项研究，以评估糖原磷酸化酶的特征。 此外，它还应用于研究肌动蛋白对不同形式肌磷酸化酶激酶的活化作用。
單位定義
在30°C、pH 7.7和ATP存在的条件下，一个单位每分钟可将磷酸化酶B转化为1.0 μ摩尔磷酸化酶。
外觀
含有（NH4）2SO4、蔗糖、β-甘油磷酸盐和二硫赤藓糖醇的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Laura A Lane et al.
Molecular & cellular proteomics : MCP, 11(12), 1768-1776 (2012-09-12)
Phosphorylase kinase (PhK), a 1.3 MDa enzyme complex that regulates glycogenolysis, is composed of four copies each of four distinct subunits (α, β, γ, and δ). The catalytic protein kinase subunit within this complex is γ, and its activity is
Monica Balsera et al.
Planta, 237(2), 619-635 (2012-12-12)
Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little
Simona Fermani et al.
The Journal of biological chemistry, 287(25), 21372-21383 (2012-04-20)
Carbon assimilation in plants is regulated by the reduction of specific protein disulfides by light and their re-oxidation in the dark. The redox switch CP12 is an intrinsically disordered protein that can form two disulfide bridges. In the dark oxidized
K F Chan et al.
The Journal of biological chemistry, 257(10), 5956-5961 (1982-05-25)
Aspects of the molecular interaction and subunit structure of rabbit skeletal muscle phosphorylase kinase, (alpha beta gamma delta)4, were investigated. Exogenous addition of the delta subunit (calmodulin) stimulated the activities of nonactivated phosphorylase kinase and the alpha gamma delta complex
G Kamp
Biological chemistry Hoppe-Seyler, 367(2), 109-117 (1986-02-01)
The activities of glycogen phosphorylases a and b from the body wall musculature of the marine worm Arenicola marina (Annelida, Polychaeta) were determined after various periods of anoxia. Already under normoxic conditions one third of the total activity was produced
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