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P0899

Sigma-Aldrich

聚-D-赖氨酸 氢溴酸盐

Poly-D-lysine hydrobromide

suitable for cell culture, Mol wt 70,000-150,000

别名:

D-Lysine homopolymer hydrobromide

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About This Item

线性分子式:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS号:
27964-99-4
MDL编号:
MFCD00131934
UNSPSC代码:
12352209
PubChem化学物质编号:
24898137
NACRES:
NA.26
价格与库存信息目前不能提供

产品名称

聚-D-赖氨酸 氢溴酸盐, mol wt 70,000-150,000

表单

solid

质量水平

200

分子量

70,000-150,000

技术

cell culture | mammalian: suitable

杂质

<10% water (Karl Fischer)

颜色

white to off-white

溶解性

water: 50 mg/mL, clear, colorless to very faintly yellow

官能团

amide

储存温度

−20°C

SMILES字符串

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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相关类别

一般描述

聚 D-赖氨酸氢溴化物 (PDLHB) 是一种水溶性、带正电的多氨基酸，在生物学和生物医学中有着广泛的应用。PDLHB 通常用于促进细胞与表面的粘附，这对于细胞培养，显微镜和神经科学研究至关重要。PDLHB 也用于组织工程和给药研究。聚-D-赖氨酸的主要功能在于促进细胞对表面的粘附，利用其正电荷通过与细胞表面带负电荷的蛋白质和分子结合来吸引和锚定细胞。这一特性在覆盖细胞培养皿和培养皿时尤其有价值，因为它对确保可靠的细胞粘附至关重要，尤其是对原代细胞和特定癌细胞等具有挑战性的细胞类型。 在显微镜和神经科学应用中，PDLHB 涂层基板走在前列，允许细胞，组织和神经元无缝附着到载玻片和培养皿上。这促进了对这些关键生物成分的细致观察和探索，使 PDLHB 成为深入研究不可或缺的资源。此外，PDLHB 通过支持细胞生长支架的创建，将其应用扩展到组织工程。这些 PDLHB 包覆支架紧密复制自然细胞环境，促进有效的愈合和再生。PDLHB 在药物传递领域的作用举足轻重，可作为包膜药物的保护屏障，精确地将它们引导到特定的细胞和组织。这种多功能和生物相容性聚合物是一种宝贵资产，提高了生物学和生物医学领域内细胞培养和生化研究的标准。

应用

多聚-D-赖氨酸氢溴酸盐被用于以下研究：
  • 动物细胞培养[1]
  • 大鼠皮层神经元培养[2]
  • 细胞培养和siRNA处理[3]
  • 原代细胞培养和成人神经元网络模型[4]
  • 皮质星形胶质细胞培养[5]
  • 免疫荧光染色（将HDF细胞接种到多聚-D-赖氨酸（P0899，Sigma-Aldrich）包被的玻璃盖玻片上）[6]
  • NPC收集和培养条件[7]
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。

建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。

生化/生理作用

多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。

特点和优势

  • 适用于细胞培养和生物化学研究
  • 适用于多种研究应用的优质化合物

组分

聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

注意

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

分析说明

本品的分子量为70,000-150,000。为了除去HBr，将本品溶解在中性缓冲液中并透析除去盐。 通常，本品用作粘附因子，将50 mL无菌组织培养级水加至5 mg多聚赖氨酸中，无菌条件下，每25 cm2 表面包被1 mL。 包被5分钟，然后吸去溶液并充分清表面。 晾干2小时，然后加入细胞和培养基。

其他说明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
如需了解生化试剂系列的更多信息，请填写此表。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000422074
0000422073
0000422072
0000422072
0000422073

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