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OGS1120

PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

Name: PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

别名:

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

分類程式碼代碼:

12352200

NACRES:

NA.85

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Slide 1 of 10

1 of 10

Sigma-Aldrich

05-6210

硫酸铜五水合物

Supelco

68279

硫酸浓缩液

Sigma-Aldrich

PP2410

Mouse IgG Vector Set

Sigma-Aldrich

72068

氢氧化钠溶液

Sigma-Aldrich

P1243

pT7-FLAG^™-2 Expression Vector

CLS3160102BO

Pyrex^® petri dishes

Sigma-Aldrich

28-2940

氢氧化钠

Sigma-Aldrich

PP2380

Bacterial Signal Peptide Vector Set

Sigma-Aldrich

28-3580

草酸钠溶液

Sigma-Aldrich

S2770

2 mol/L 氢氧化钠溶液溶液

籤條

10-His tagged

形狀

buffered aqueous solution

分子量

size 6201 bp

菌種選擇

kanamycin

哺乳動物細胞選擇

puromycin

複製起點

pUC (500 copies)

肽切割

TEV

肽標籤位置

C-terminal

啟動子

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

報告基因

none

運輸包裝

ambient

儲存溫度

−20°C

一般說明

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal Deca-Histidine (10His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

序列

To view sequence information for this product, please visit the product page

分析報告

To view the Certificate of Analysis for this product, please visit www.oxgene.com

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.

Alexander C Cerny et al.

PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)

Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

In vivo characterization of the physicochemical properties of polymer-linked TLR agonists that enhance vaccine immunogenicity.

Geoffrey M Lynn et al.

Nature biotechnology, 33(11), 1201-1210 (2015-10-27)

The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer

Dickkopf-3 regulates prostate epithelial cell acinar morphogenesis and prostate cancer cell invasion by limiting TGF-β-dependent activation of matrix metalloproteases.

Diana Romero et al.

Carcinogenesis, 37(1), 18-29 (2015-10-28)

Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we

Notch3 interactome analysis identified WWP2 as a negative regulator of Notch3 signaling in ovarian cancer.

Jin-Gyoung Jung et al.

PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)

The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.

商品

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

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Key Documents

PSF-CMV-PURO-COOH-TEV-10HIS - C-TERMINAL 10HIS TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

About This Item

推荐产品

属性

籤條

形狀

分子量

菌種選擇

哺乳動物細胞選擇

複製起點

肽切割

肽標籤位置

啟動子

報告基因

運輸包裝

儲存溫度

说明

一般說明

序列

分析報告

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安全信息

儲存類別代碼

閃點（°F）

閃點（°C）

文件

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