For assay purposes, this product is reconstituted at 0.02 - 0.04 u/mL in water containing 0.2% bovine serum albumin (BSA). The enzyme may also be prepared as follows:
Water containing 0.2% BSA at 2 mg/mL yielding a clear tan or brown solution
10 mM phosphate buffer, pH 6, containing 25 to 150 mM KCl at 120ug/mL. This stock solution may be stored refrigerated for several months.
价格与库存信息目前不能提供
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品質等級
種類
Type V
形狀
lyophilized powder
比活性
≥0.1 units/mg solid (using mucin)
≥1.3 units/mg solid (using 4MU-NANA)
應用
diagnostic assay manufacturing
異物活動
Protease and NAN-aldolase, present
運輸包裝
dry ice
儲存溫度
−20°C
基因資訊
Clostridium perfringens str. 13 ... nanI(988807)
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相关类别
一般說明
神经氨酸酶是一种水解酶，其可促进流感病毒从感染细胞释放并促进病毒的传播。
生化／生理作用
使用神经氨酸酶去除细胞表面上糖蛋白的唾液酸残基已有多次报道。通常，实验步骤中会表明将神经氨酸酶在PBS中，37℃条件下孵育30分钟进行使用，然后用PBS洗涤几次。用浓度低得多的神经氨酸酶处理组织切片需要更长的温育时间：在pH 4.2-5的0.1M乙酸盐缓冲液中，对于1-4U/mL浓度，需要在37℃下孵育2-20小时。
利用神经氨酸酶裂解唾液酸基团已用来研究糖蛋白结构的抗体识别。与其他两种方法进行比较，使用神经氨酸酶可更好的对N-乙酰神经氨酸进行估测。
神经氨酸酶可以通过去除细胞表面上暴露的带负电荷的唾液酸残基来增加某些细胞系中的聚集。 [3]
神经氨酸酶可用于从多种糖蛋白中切割末端N-乙酰神经氨酸（唾液酸）。 来自产气荚膜梭菌的酶可切割末端唾液酸残基，这些残基可通过α-2,3-、α-2,6-或α-2,8-与Gal、GlcNac、GalNAc、AcNeu、GlcNeu、寡糖、糖脂或糖蛋白进行连接。切割的相对速率按以下顺序逐渐降低：α-2-3>α-2-6。α-2-8。与来自A. ureafaciens的神经氨酸酶（Sigma N3642）（优先切割α-2-6连接的残基）相比，来自产气荚膜梭菌的神经氨酸酶可最有效地切割α-2-3连接的唾液酸残基。
單位定義
在37°C、pH 5.0的条件下，一个单位每分钟可从牛下颌粘蛋白中释放1.0微摩尔的N-乙酰神经氨酸。以4MU-NANA为底物，
一个单位在37°C、pH 5.0的条件下，每分钟可释放1.0微摩尔的2′-（4-甲基伞形酮）-a-D-N-乙酰基神经氨酸。
一个单位在37°C、pH 5.0的条件下，每分钟可释放1.0微摩尔的2′-（4-甲基伞形酮）-a-D-N-乙酰基神经氨酸。
準備報告
其通过盐分馏进行制备。
分析報告
按照4MU-NANA单位进行包装
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes.
M S Weiner et al.
Blood, 42(6), 939-946 (1973-12-01)
Randa Bittar et al.
Practical laboratory medicine, 18, e00150-e00150 (2020-01-08)
A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also repeatability and reproducibility of the method
J J Deman et al.
The Journal of cell biology, 60(3), 641-652 (1974-03-01)
Aggregation of suspended HeLa cells is increased on removal of cell surface sialic acid. Calcium ions promote aggregation whereas magnesium ions have no effect. The calcium effect is abolished by previous treatment of the cells with neuraminidase. Trypsinization of the
S Gatt et al.
The Biochemical journal, 193(1), 267-273 (1981-01-01)
Studies were done on the effect of bile salts on the rates of hydrolysis of the N-acetylneuraminyl linkages of several sialic acid-containing compounds by the neuraminidase of Clostridium perfringens. When GM3-ganglioside, two glycolipids (glycophorin and orosomucoid) and neuraminyl-lactose were used
Francesca Ferrara et al.
Frontiers in immunology, 12, 661379-661379 (2021-06-11)
Influenza B viruses (IBV) cause respiratory disease epidemics in humans and are therefore components of seasonal influenza vaccines. Serological methods are employed to evaluate vaccine immunogenicity prior to licensure. However, classical methods to assess influenza vaccine immunogenicity such as the
商品
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
实验方案
Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.
在测定神经氨酸酶活性时，使用了一种以N-乙酰新胺酸为酶底物的比色法进行测定。神经氨酸酶是一种可促进流感病毒从感染细胞中释放的水解酶。
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What should I use to dilute this product?
1 answer-
Helpful?
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Does this enzyme cleave Neu5Gc?
1 answer-
Product N2876, Neuraminidase, will cleave α2-3, α2-6, and α2-8 glycosidic linkages. Neu5Gc are mostly found terminally on glycan chains of glycoproteins and glycolipids. They are commonly linked via an α2-3 or α2-6 linkage to Gal, an α2-6 linkage to GalNAc, or via an α2-8 linkage to another sialic acid.
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What is the gene sequence for this product?
1 answer-
See the link below to review the gene information of Clostridium perfringens str. 13 nanI(988807) on NCBI:
https://www.ncbi.nlm.nih.gov/gene/988807The specific sequence of the orgnism use in the production of this product is considered proprietary.
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How should lyophilized neuraminidase be resuspended for storage in aqueous form? I plan to introduce it to cell culture.
1 answer-
The recommendation is to dissolve in water containing 0.2% BSA (albumin) at 2 mg/mL. This will yield a clear tan or brown solution. Neuraminidase is a comparatively stable enzyme in solution. When dissolved at 120 µg/mL in 10 mM phosphate buffer, pH 6, containing 25 to 150 mM KCl, the enzyme retained activity at 0-4 C for over 30 months. At a concentration of 2 µg/mL under the same conditions, activity was lost unless albumin (BSA) was added.
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