推荐产品
一般描述
应用
特点和优势
- 载体: U6-gRNA/PGK-Puro-2A-BFP(仅gRNA)
- 简化嘌呤霉素选择的流程
- 用BFP点亮表达CRISPR的细胞
- 使用转座酶将表达成分翻转进入或移出基因组
其他特点
- 更好而不是更大:每个人类基因配有两个优化的克隆,从而减少了筛选实验的时间、成本和规模
- 可直接用于筛选:克隆已被加入便于机器人操作的96孔形式中,从而进行高通量筛选。需要进行质粒制备以转染细胞或慢病毒制备以转导细胞
- 协作:实时,连续文库验证
有关Sanger文库的详细信息, 请点击此处
包装
组分
外形
其他说明
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
商品
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
全基因组功能缺失筛查是发现生物过程背后的基因和途径的有效方法。现在,每个基因都可通过两个优化的 gRNA 完全敲除。通过最大限度减少克隆数量,可确保尽可能特异性的筛选,同时控制时间和成本。
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
实验方案
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
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