CRISPR18

Lenti CRISPR Universal Non-Target Control#1 Plasmid DNA (LV04 vector)

• 分類程式碼代碼: 41106609
• NACRES: NA.51

属性

• 形狀: liquid
• 品質等級: 200
• 包裝: vial of 50 μL
• 濃度: 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

應用

功能性基因组学研究/筛选/靶标验证

其他說明

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).

法律資訊

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable