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用途
sufficient for 200 tests (UV)
特點
This kit is sufficient for up to 100 assays of 3-hydroxybutyric acid (BOH) and up to 100 assays of acetoacetic acid (AcAc) in 96 well plates.
檢測方法
colorimetric
相關疾病
endocrinological disorders, diabetes; neonatal diseases; pediatric diseases; cancer
儲存溫度
−20°C
一般說明
酮体 3-羟基丁酸 (BOH) 和乙酰乙酸 (AcAc) 主要在肝脏中由脂肪酸氧化生成 ，正常情况下在尿液和血液中浓度较低。血液中酮体浓度升高可能导致代谢性酸中毒，这与糖尿病、儿童期低血糖、生长激素缺乏症、酒精或水杨酸盐中毒以及先天性代谢缺陷相关。
應用
酮体测定法已用于测量人肝癌细胞系 HepG2 培养基中酮体的释放。
特點和優勢
兼容高通量的操作系统。
適合性
可用于检测血清、血浆、尿液及其他生物样品中的酮体（乙酰乙酸和3-羟基丁酸）。
原則
在本试剂盒中，使用基于 3-羟基丁酸脱氢酶催化反应的酶分析法测定 AcAc 和 BOH 水平，其中在 340 nm 处测定的 NADH 吸光度更改与 AcAc 和 BOH 浓度直接相关。
儲存類別代碼
10 - Combustible liquids
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