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Merck

M6770

Sigma-Aldrich

MCDB 201 培养基

With trace elements, L-glutamine and 30 mM HEPES, powder, suitable for cell culture

别名:

MCDB medium

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About This Item

分類程式碼代碼:
41161501
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

成分

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

MCDB 201培养基是为鸡胚成纤维细胞克隆生长而合成的改良Ham营养混合物F-12。

應用

MCDB 201培养基已用作以下材料的组分:
  • 培养多能干细胞的肝分化培养基
  • 培养前脂肪细胞的附睾生长培养基
  • 用于培养人脐带间充质干细胞的杜氏改良Eagle培养基(DMEM))/F12

數量

配制成每升培养基中含 17.7 克粉末。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Jeff Ishibashi et al.
Molecular and cellular biology, 32(12), 2289-2299 (2012-04-05)
Fibroblastic preadipocyte cells are recruited to differentiate into new adipocytes during the formation and hyperplastic growth of white adipose tissue. Peroxisome proliferator-activated receptor γ (PPARγ), the master regulator of adipogenesis, is expressed at low levels in preadipocytes, and its levels
Baris Ulum et al.
Journal of cellular physiology, 233(11), 8429-8436 (2018-05-26)
Bone marrow mesenchymal stem cells (BM-MSCs) are promising candidates for regenerative medicine purposes. The effect of obesity on the function of BM-MSCs is currently unknown. Here, we assessed how obesity affects the function of BM-MSCs and the role of endoplasmic
Philip Roelandt et al.
Methods in molecular biology (Clifton, N.J.), 997, 141-147 (2013-04-03)
Differentiation of human stem cells to hepatocytes is crucial for industrial applications as well as to develop new therapeutic strategies for liver disease. The protocol described here, using sequentially growth factors known to play a role in liver embryonic development
Philip Roelandt et al.
PloS one, 5(8), e12101-e12101 (2010-08-17)
Stem cell-derived hepatocytes may be an alternative cell source to treat liver diseases or to be used for pharmacological purposes. We developed a protocol that mimics mammalian liver development, to differentiate cells with pluripotent characteristics to hepatocyte-like cells. The protocol
Jeff Ishibashi et al.
Molecular and cellular biology, 32(12), 2289-2299 (2012-04-05)
Fibroblastic preadipocyte cells are recruited to differentiate into new adipocytes during the formation and hyperplastic growth of white adipose tissue. Peroxisome proliferator-activated receptor γ (PPARγ), the master regulator of adipogenesis, is expressed at low levels in preadipocytes, and its levels

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