Product No. D6046 is the fully supplemented liquid form of the original Dulbecco's formulation, offered as Product No. D5523, with one key exception. Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. D5546 is the same but does not contain L-glutamine.
推荐产品
品質等級
形狀
powder
技術
cell culture | mammalian: suitable
成分
L-glutamine: yes
NaHCO3: no
sodium pyruvate: yes
phenol red: yes
HEPES: no
glucose: low
運輸包裝
ambient
儲存溫度
2-8°C
正在寻找类似产品? 访问 产品对比指南
一般說明
杜氏改良Eagle培养基（DMEM）是对Eagle基础培养基 (BME)的改良，含有四倍浓度的氨基酸和维生素以及其他补充组分。据报告首个DME配方用于培养含有1,000 mg/L葡萄糖的胚胎小鼠细胞。4,500 mg/L葡萄糖的修饰对培养某些类型的细胞效果最佳。
應用
杜氏改良Eagle低葡萄糖培养基已用于人和小鼠间充质干细胞（MSC）的分化和培养。[1]
數量
配制成每升培养基含 10.0 克粉末。
重構
补充 3.7 g/L 碳酸氢钠。
也與該產品經常一起購買
产品编号
说明
价格
補充物
产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Daniela Steffens et al.
Journal of biomaterials applications, 32(5), 663-676 (2017-10-11)
Laminin-functionalized poly-d,l-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were implanted. The analysis is comprised of the visual aspect and mean
Akon Higuchi et al.
Scientific reports, 5, 10217-10217 (2015-05-15)
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs
Gayatri Jamwal et al.
Biochimica et biophysica acta, 1865(6), 920-931 (2018-04-06)
IGF1R is a ubiquitous receptor tyrosine kinase that plays critical roles in cell proliferation, growth and survival. Clinical studies have demonstrated upregulation of IGF1R mediated signaling in a number of malignancies including colon, breast, and lung cancers. Overexpression of the
Zikai Feng et al.
Pharmaceuticals (Basel, Switzerland), 13(2) (2020-02-16)
Short-chain quinones (SCQs) have been identified as potential drug candidates against mitochondrial dysfunction, which is largely dependent on their reversible redox characteristics of the active quinone core. We recently synthesized a SCQ library of > 148 naphthoquinone derivatives and identified
Kallyne K Mimura et al.
Molecular vision, 18, 1583-1593 (2012-06-29)
The aim of this study was to evaluate the expression of the protein annexin A1 (ANXA1), a potent endogenous regulator of the inflammatory process, in ocular toxoplasmosis. C57BL/6 female mice were infected using intravitreal injections of either 10(6) tachyzoites of
实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
-
Do you offer a liquid version of Dulbecco’s Modified Eagle’s Medium - low glucose?
1 answer-
Helpful?
-
-
How do I store Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose, dry powder? How do I store the reconstituted medium?
1 answer-
Store the dry powdered medium at 2-8 °C under dry conditions and liquid medium at 2-8 °C in the dark. The nature of supplements added may affect storage conditions and shelf life of the medium. The product label bears the lot-specific expiration date.
Helpful?
-
-
How do I prepare Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
1 answer-
Measure out 90% of final required volume of water. Water temperature should be 15-20 °C.While gently stirring the water, add the powdered medium. Stir until dissolved. Do NOT heat.Rinse original package with a small amount of water to remove all traces of powder. Add to solution in step 2.To the solution in step 3, add 3.7 g sodium bicarbonate or 49.3 ml of sodium bicarbonate solution [7.5%w/v] for each liter of final volume of medium being prepared. Stir until dissolved.While stirring, adjust the pH of the medium to 0.1-0.3 pH units below the desired pH since it may rise during filtration. The use of 1N HCl or 1N NaOH is recommended.Add additional water to bring the solution to final volume.Sterilize immediately by filtration using a membrane with a porosity of 0.22 microns.Aseptically dispense medium into sterile container.
Helpful?
-
-
What is the formulation of Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
1 answer-
The formulation can be found on the DMEM formulation sheet at the following link.
Helpful?
-
-
What sodium bicarbonate should I use with Product D5523, Dulbecco’s Modified Eagle’s Medium - low glucose?
1 answer-
Sodium Bicarbonate powder (Product No. S5761) or Sodium Bicarbonate Solution, 7.5% (Product No. S8761) is recommended. For more information, consult the product datasheet.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门
