推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
MDC1-50, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~250 kDa (2-3 bands)
物種活性
monkey, human
包裝
antibody small pack of 25 μL
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of G361 cells
同型
IgG2a
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... MDC1(9656)
一般說明
Monoclonal Anti-MDC1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a recombinant human MDC1 fragment. Mediator of DNA damage checkpoint protein 1 (MDC1) contains 2089 amino-acid residues with a predicted molecular weight of 226.4 kDa. The protein contains an FHA (forkhead-associated) domain at its amino terminus and two BRCT (BRCA1 carboxyl terminal) domains at its carboxy terminus.
特異性
Mouse monoclonal clone MDC1-50 anti-MDC1 antibody recognizes human and monkey MDC1.
免疫原
recombinant human MDC1 fragement (amino acids 2-200).
應用
Monoclonal Anti-MDC1 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA
- immunofluorescence
- western blotting
- immunoprecipitation
- immunocytochemistry
Mouse monoclonal clone MDC1-50 anti-MDC1 antibody is used to tag mediator of DNA damage checkpoint protein 1 for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques such as ELISA, immunoblotting (approx. 250 kDa, 2-3 bands), immunoprecipitation and immunocytochemistry. It is used as a probe to determine the roles of mediator of DNA damage checkpoint protein 1 in DNA repair and genomic stability.
生化／生理作用
Genomic instability caused by the disruption of mechanisms that regulate cell-cycle checkpoints, DNA repair and apoptosis may lead to the development of cancer. ATM and ATR protein kinases are mediated to DNA damage sites by molecular adapters or mediators proteins such as Histone H2AX, Claspin and BRCTmotif containing molecules such as 53BP1, BRCA1, and MDC1 (mediator of DNA damage checkpoint protein 1). MDC1 interacts with the MRE11 complex (containing MRE11, RAD50 and NBS1 proteins). The MRE11 complex is involved in the detection repair and signaling of DNA damage. Upon ionizing radiation MDC1 is hyperphosphorylated by ATM and localizes to nuclear foci together with the MRE11 complex, phosphorylated H2AX and 53BP1. A radio resistant DNA synthesis (RDS) phenotype in cells is formed by down regulation of MDC1 protein expression by siRNA.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Helen C Turner et al.
Radiation and environmental biophysics, 53(2), 265-272 (2014-01-31)
At the Center for High-Throughput Minimally Invasive Radiation Biodosimetry, we have developed a rapid automated biodosimetry tool (RABiT); this is a completely automated, ultra-high-throughput robotically based biodosimetry workstation designed for use following a large-scale radiological event, to perform radiation biodosimetry
53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress
Lukas C, et al.
Nature Cell Biology, 13(3), 243-243 (2011)
DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors
Francia S, et al.
Journal of Cell Science, 129(7), 1468-1476 (2016)
A dual interaction between the DNA damage response protein MDC1 and the RAG1 subunit of the V (D) J recombinase
Coster G, et al.
The Journal of biological chemistry, 287(43), 36488-36498 (2012)
Zi-Zhen Zhang et al.
World journal of gastroenterology, 19(12), 1968-1974 (2013-04-10)
To investigate the expression deficiency of key molecular markers in the homologous recombination pathway. Expression loss of breast cancer type 1 susceptibility protein (BRCA1), ataxia telangiectasia mutated (ATM), ATM-Rad3-related (ATR), mediator of DNA damage checkpoint protein 1 (MDC1) and meiotic
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门