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L6420

Sigma-Aldrich

黄体激素 来源于人类脑垂体

≥8,500 IU/mg

别名:

ICSH, LH, PLH

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.77
价格与库存信息目前不能提供

质量水平

比活

≥8,500 IU/mg

运输

dry ice

储存温度

−20°C

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生化/生理作用

双链糖蛋白激素;α-链与绒毛膜促性腺激素、TSH 和 FSH 的相同,而生物学特异性归因于独特的 β-链。诱导排卵、精子生成和类固醇性激素的合成。
双链糖蛋白激素;α-链与绒毛膜促性腺激素、TSH 和 FSH 的相同,而生物学特异性归因于独特的 β-链。诱导排卵、精子生成和类固醇性激素的合成。活性是通过跨膜 LH/绒毛膜促性腺激素受体介导的,该受体是 G 蛋白偶联受体(GPCR)家族的成员。

外形

从 50 mM 碳酸氢铵中冻干

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说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Mitsuru Komeya et al.
Scientific reports, 6, 21472-21472 (2016-02-20)
In contrast to cell cultures, particularly to cell lines, tissues or organs removed from the body cannot be maintained for long in any culture conditions. Although it is apparent that in vivo regional homeostasis is facilitated by the microvascular system
Hanyong Jin et al.
Biochemical and biophysical research communications, 482(4), 877-882 (2016-11-29)
Pituitary gonadotropins are key hormones that orchestrate the growth and development of ovarian follicles. However, limited information is available on intra-ovarian factors that mediate the actions of gonadotropins. In this study, we identified that the early growth response 2 gene
Affiefa Yawer et al.
Toxicology and applied pharmacology, 404, 115177-115177 (2020-08-03)
A decline in male fertility possibly caused by environmental contaminants, namely endocrine-disrupting chemicals (EDCs), is a topic of public concern and scientific interest. This study addresses a specific role of testicular gap junctional intercellular communication (GJIC) between adjacent prepubertal Leydig
Cheng Peng et al.
General and comparative endocrinology, 267, 90-97 (2018-06-19)
Luteinizing hormone receptor (LHR) plays a critical role in reproduction by mediating LH signaling in the gonad. In this study, we cloned a novel lhr gene from the orange-spotted grouper, named glhr2. The cloned complete open reading frame sequence of
Xuerui Yao et al.
Theriogenology, 151, 66-73 (2020-04-21)
Zearalenone (ZEA), which is produced by several fusarium mycotoxins, is found in animal feed and food products, and can exert estrogen-like activity. Melatonin (MT) is emerging as a supplement that can fight the toxic effects of mycotoxins. With a variety

Questions

1–5 of 5 Questions  
  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Could you provide the concentration of L6420 in nanograms per milliliter (ng/ml)?

    1 answer
    1. Product L6420 is a white powder that is lyophilized from a 50 mM ammonium bicarbonate solution. Since this product is not a solution, it does not have an associated concentration in ng/mL.

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  4. What volumen of the buffer should be added to dilute the product?

    1 answer
    1. For a stock solution, hormones should be reconstituted to ≥1 mg/ml in the presence of a protease-free carrier (0.1-1% ovalbumin, BSA, or gelatin) and a suitable antimicrobial agent. The following general guidelines may be useful:

      1. Ovalbumin is generally used when assaying for gonadotropin;
      2. If BSA is used, it must be free of gonadotropins and proteases (either crystalline or RIA grade is generally satisfactory);
      3. Heat gelatin solutions to 60°C for maximum solubility (1% gelatin for ELISAs, 0.1% for solid phase assays);
      4. For the pituitary hormones hFSH, hLH, hPRL, and hTSH, the recommended dilution buffer is 10 mM Sodium Phosphate, 150 mM Sodium Chloride, 1 mg/ml BSA, 0.1% Sodium Azide, pH 7.4 is generally satisfactory for hFSH, hLH, hPRL, and hTSH.
      5. For hCG and hGH, the recommended dilution buffer is 100 mM Tris, 1 mg/ml BSA, 0.05% Sodium Azide, pH 8.0 is generally satisfactory for hCG and hGH.

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  5. How to dilute the LH L6420

    1 answer
    1. It is recommended to dilute Product LH L6420 in a buffer consisting of 10 mM Sodium Phosphate, 150 mM Sodium Chloride, 1 mg/ml BSA, 0.1% Sodium Azide (optional), pH 7.4.

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