KCQS05

KiCqStart^® Probe qPCR ReadyMix^™，低ROX^™

with Low ROX for ABI and Stratagene instruments

• 别名: Kicqstart^® Probe qPCR ReadyMix^™
• 分類程式碼代碼: 41106300
• NACRES: NA.55

属性

• 形狀: liquid
• 用途: sufficient for 1250 reactions; sufficient for 250 reactions; sufficient for 5000 reactions
• 特點: dNTPs included; hotstart
• 儲存條件: protect from light
• 技術: qPCR: suitable
• 顏色: light blue
• 輸入: purified DNA
• 相容性: ABI 7500 Fast; ABI 7500; ABI QuantStudio; ABI ViiA 7 ; Agilent AriaMx; Douglas Scientific IntelliQube; Qiagen Corbett Rotor-Gene Q; Strategene Mx3000P; Strategene Mx3005P; Strategene Mx4000
• 檢測方法: probe-based
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

KiCqStart^® Probe qPCR ReadyMix^™ is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. KiCqStart Probe qPCR ReadyMix is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue color of an inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

A key component of KiCqStart Probe qPCR ReadyMix is an ultra-pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA§. This PCR master mix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. The enzyme in KiCqStart Probe qPCR ReadyMix is combined with high avidity monoclonal antibodies to provide a stringent automatic hot-start that allows reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

應用

KiCqStart^® Probe qPCR ReadyMix^™, Low ROX^™ has been used as a component of reaction mixture for detection of genomic DNA extracted from parasites by multiplex quantitative polymerase chain reaction (qPCR) and Real-Time PCR (qPCR).
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.

特點和優勢

• 仅需33分钟即可获得检测结果
• 高效和高灵敏度的实时PCR结果
• 极少/无需优化

成分

2X reaction buffer containing optimized concentrations of MgCl₂, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

法律資訊

KiCqStart is a registered trademark of QIAGEN Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable