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Merck
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I1146

Sigma-Aldrich

R3 IGF-1 人

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

别名:

Human IGF-1, IGF-1 Protein, Recombinant IGF-1

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About This Item

分類程式碼代碼:
51111800
NACRES:
NA.77
价格与库存信息目前不能提供

生物源

human

品質等級

200

重組細胞

expressed in E. coli

化驗

≥85% (HPLC)

形狀

lyophilized powder

效力

≤10 ng/mL ED50

品質

endotoxin tested

分子量

7,676 Da

包裝

pkg of 0.1 mg

技術

cell culture | mammalian: suitable

UniProt登錄號

P05019

儲存溫度

2-8°C

基因資訊

human ... IGF1(3479)

相关类别

應用

R3 IGF-I 是胰岛素样生长因子的重组类似物，包含完整的人 IGF-I 氨基酸序列，其中 Arg 取代了 Glu3。它被开发为一种廉价、高质量、高效的 IGF 类似物，可用作无血清或低血清细胞培养的生长因子补充剂。

使用专利表达系统在大肠杆菌中重组生产，该肽是从溶解并脱盐的包含体的细胞中收获。活性分子在氧化条件下适当折叠，然后通过多个液相色谱步骤纯化。

生化／生理作用

中国仓鼠卵细胞(CHO)的增殖。

其他說明

胰岛素样生长因子-I 的重组类似物，在第 3 位用 Arg 取代 Glu。

準備報告

由0.1 M醋酸制备

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000352239
0000337520
0000375052
0000352239
0000337520

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商品

Insulin-like Growth Factors (IGF)

Insulin-like Growth Factors (IGF)

Questions

1–2 of 2 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. How do the sequences of Products I1146 and I1271 compare to that of native Insulin-like Growth Factor 1 (IGF-1)?

    1 answer

    1. The entire IGF-1 gene codes for 194 amino acids, but the mature form of the protein is 70 amino acids, consisting of amino acids 49-118.Product I1146 is R3 IGF-1, which is also 70 amino acids in length. The Glutamic acid (E) at position 3 has simply been replaced by an Arginine (R). So the sequence of Product I1146 is:GP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSAProduct I1271 is the same as I1146, but with an additional thirteen amino acids "in front of" the normal N-terminus of the R3 IGF-1:MFPAM PLSSLFVNGP RTLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSANote that the first 13 amino acids in the recombinant I1271 (MFPAMPLSSLFVN)are quite different from what would have been the preceding 13 amino acids in the immature IGF-1.

      Helpful?

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