推荐产品
ligand
dextrin
包裝
pack of 5 × 5 mL
製造商/商標名
Cytiva 28-9187-80
儲存條件
(20% Ehtanol)
參數
<20 mL/min flow rate
42 psi
床尺寸
16 mm × 25 mm
床體積
5 mL
柱內徑
16 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-13
工作範圍
>7
容量
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
儲存溫度
2-8°C
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一般說明
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
特點和優勢
- HiTrap® columns prepacked with Dextrin Sepharose™ High Performance medium.
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
- Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
- Physiological conditions and mild elution preserve the activity of the target protein.
- Convenient, time-saving operation and reproducible resultsLab Purification using the affinity to the MBP tag.
儲存和穩定性
分析報告
其他說明
法律資訊
訊號詞
WarningDanger
危險聲明
危險分類
Flam. Liq. 2
儲存類別代碼
3 - Flammable liquids
水污染物質分類(WGK)
WGK 1
其他客户在看
商品
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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