推荐产品
ligand
recombinant protein G lacking albumin-binding region
材料
polypropylene and polyethylene
特點
wetted part: no
包裝
pkg of 4 ea
製造商/商標名
Cytiva 28-9031-35
儲存條件
(20% Ehtanol)
(20% Ethanol)
參數
30 °C max. temp.
4 °C min. temp.
尺寸
127.8 mm × 85.5 mm × 30.6 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-9
工作範圍
3-9
容量
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
儲存溫度
2-8°C
一般說明
Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).
A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
特點和優勢
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, protocol for classical elution as well as cross-linking method.
- Formatted for elution for electrophoresis or LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein G HP prepacked columns.
- Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
其他客户在看
商品
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
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