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GE17-0404-01

HiTrap® Protein G High Performance

Cytiva 17-0404-01, pack of 5 × 1 mL

别名:

Protein purification column

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About This Item

UNSPSC代码:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

表单

prepacked column

交联

cross-linked

包装

pack of 5 × 1 mL

制造商/商品名称

Cytiva 17-0404-01

储存条件

(20% Ehtanol)
 (20% Ethanol)

参数

<4 mL/min flow rate
42 psi

技术

liquid chromatography (LC): suitable

柱床尺寸

7 mm × 25 mm

柱床体积

1 mL

色谱柱内径

7 mm

尺寸

基质

(cross-linked agarose, spherical)

基质活性基团

Protein G functional group

平均零件尺寸

34 μm

cleaning

2-9

工作范围

3-9

容量

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

分离技术

affinity

储存温度

2-8°C

相关类别

一般描述

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特点和优势

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

储存及稳定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析说明

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他说明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律信息

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形图

Flame
GHS02

警示用语：

Warning

危险声明

H226

储存分类代码

3 - Flammable liquids

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Lin-Ping Wu et al.
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Complement is an enzymatic humoral pattern-recognition defence system of the body. Non-specific deposition of blood biomolecules on nanomedicines triggers complement activation through the alternative pathway, but complement-triggering mechanisms of nanomaterials with dimensions comparable to or smaller than many globular blood

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实验方案

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This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

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This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap® Protein G HP from Cytiva for the capture step.

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