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技術
protein array: suitable
基質活性組
phase
膨潤
1 g swells to 2-3 mL
應用
life science and biopharma
相容性
Cytiva
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一般說明
Sephadex G-10葡聚糖凝胶是常见的凝胶过滤树脂，专门设计用于多肽和生物小分子的脱盐和缓冲液置换。该型号具有Sephadex中最小的排阻极限，令其在这些应用中十分有效。Sephadex是葡聚糖与环氧氯丙烷交联制备而成的凝胶过滤树脂。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分级范围也不同。Sephadex G-10属于G型，G型Sephadex的型号包括从G-10（用于小分子）到G-75（用于大分子）的5种型号。
應用
Sephadex® G-10可作为体积排阻材料用于将标记的N-聚糖与多余的染料分离。还可用于脱盐以降低盐浓度用于阴离子交换(AEx)和阳离子交换色谱(CEx)。
Sephadex® G-10已被用于将膜蛋白重建为巨大的蛋白脂质体。
生化／生理作用
Sephadex G-10是凝胶过滤介质，用于凝胶过滤色谱和蛋白质色谱。应用广泛，已被用于癌症研究，并可用于测定自来水、河水和绿茶等多种样品中的氟化物含量。
特點和優勢
- 一步完成脱盐、除污染和置换新缓冲液。
- 适合用于缓冲液置换和生物样品纯化。
- 适合分离分子量超过700的多肽、小分子蛋白和多糖。
- 高效去除盐、染料和放射性标记。
- 具有Sephadex G型中最低的700分子量排阻极限
法律資訊
Sephadex is a registered trademark of Cytiva
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
H Han et al.
Nucleic acids research, 22(14), 2837-2844 (1994-07-25)
Different helical conformations of DNA (D), RNA (R), and DNA.RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA.Fe to a single nucleotide on RNA as well as DNA
M Muirhead et al.
International journal of cell cloning, 8(6), 392-408 (1990-11-01)
We have cloned a stromal cell from mouse bone marrow selected on the basis of its ability to promote the growth of an Abelson virus-transformed pre-B cell line. These stromal cells have smooth muscle features, and the ultrafiltrate of stromal
Kwang Wook Hyun et al.
Peptides, 27(6), 1173-1178 (2005-11-18)
This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found
Miniaturized parallel screens to identify chromatographic steps required for recombinant protein purification
Rege K and Heng M
Nature Protocols (2010)
Kaushal Rege et al.
Nature protocols, 5(3), 408-417 (2010-03-06)
Methods development in chromatography is a time-consuming, trial-and-error process that requires laborious experimentation. We describe a high-throughput screening (HTS) protocol for the rapid identification of chromatographic steps for protein purification from cell-free expression broths. Broths containing the protein are loaded
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