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FD4

Sigma-Aldrich

异硫氰酸荧光素-葡聚糖

average mol wt 3,000-5,000

别名:

FITC-右旋糖酐

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About This Item

CAS号:
60842-46-8
MDL编号:
MFCD00131092
UNSPSC代码:
12352201
NACRES:
NA.25
价格与库存信息目前不能提供

生物来源

synthetic

质量水平

100

偶联物

FITC conjugate

表单

powder

分子量

average mol wt 3,000-5,000

标记范围

0.001-0.020 mol FITC per mol glucose

技术

HPLC: suitable

颜色

light yellow to dark orange

溶解性

H2O: 25 mg/mL

适用性

suitable for HPLC
suitable for fluorescence indicator
suitable for fluorescent labeling techniques

应用

life science and biopharma
metabolomics
microbiology
pharmaceutical

储存温度

2-8°C

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相关类别

一般描述

FITC-葡聚糖也称异硫氰酸荧光素标记葡聚糖，它是一种多功能的分子标志物，广泛应用于细胞和组织研究，包括生物化学研究。该化合物是 FITC 荧光染料与葡聚糖连接的产物，葡聚糖是由不同长度的支链葡萄糖分子组成的多糖。FITC-葡聚糖有助于测定细胞和组织渗透性，它提供关键的定量数据，并且是生物医学和生物化学研究中必不可少的试剂。

在涉及血脑屏障（BBB）的生物化学研究中，FITC-葡聚糖，尤其是平均分子量4000的FITC-葡聚糖，起着核心作用。它是评估穿透血脑屏障的溶质、离子和蛋白质渗透性的必不可少的工具，有助于增进人们对屏障完整性和任何潜在破裂的了解。 这对生物化学研究的贡献非常巨大，提供了有关大脑防御机制的信息。

此外，FITC-葡聚糖在生物化学研究领域有关细胞渗透性的研究中起关键作用。这种应用还涵盖了BBB渗透性和BBB破裂程度的评估，这两方面是神经科学和药物递送研究的基础。这些生物化学研究领域对于理解疾病机制和设计有效治疗策略必不可少。

此外， FITC-葡聚糖是心血管、微循环和灌注研究的基石。它可用作荧光通量示踪化合物，可实现对血流、膜完整性、血管引流和肾脏清除等关键过程的检测。总而言之，FITC-葡聚糖是生物学、生物化学和医学领域中必不可少的工具，提供了有关细胞和组织渗透性的复杂性、血脑屏障完整性和理解多种生理病理过程的的前所未有的信息。

应用

FITC-葡聚糖已用于心血管、微循环、灌注细胞单层和细胞膜通透性研究，作为可支持对血流、膜损伤、血管引流和肾脏消除等过程进行测量的荧光通量示踪剂化合物。 小的FITC-葡聚糖已用于对内吞作用和细胞连接渗透性等过程进行测量。 5kDa的FITC-葡聚糖可用于对微尺度扩散测量进行校准。 5kDa的FITC-葡聚糖可用于在肠屏障功能障碍研究中对肠道通透性进行测量。
FITC-葡聚糖被广泛用于利用微荧光法对微循环和细胞渗透性的研究。 FITC-葡聚糖已用于研究植物细胞壁的孔隙率和毛细管渗透性。血浆蛋白已被显示不可与FITC-葡聚糖进行结合。
异硫氰酸荧光素标记的葡聚糖已用于：
  • 作为膜渗透性研究中的示踪剂分子[1]
用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。

生化/生理作用

FITC-葡聚糖是一种在生物学和生理学研究中得到广泛应用的、多功能荧光示踪剂。其主要作用包括评估生物屏障的渗透性，如血脑屏障、细胞膜和血管壁。它也用于研究细胞摄取机制、内噬、细胞连接（cell junction）渗透性，这些研究主要关注基本的细胞过程。FITC-葡聚糖有助于测定血流、血管引流（vascular drainage）和肾清除，提供有关微循环和毛细管动力学的信息。此外，这种标志物也有助于理解组织和器官特性，并可作为追踪分子的重要工具，为探索各种生物化学现象做出贡献。

特点和优势

  • 适用于生物化学和生物医学研究
  • 用途广泛，适用于各种实验室和研究应用

制备说明

Sigma通常在水中以25 mg/ml或更低的浓度对FITC葡聚糖的溶解度进行检测。 溶液应当避光保存。 FITC-葡聚糖在体外可稳定超过24小时。

其他说明

为了全面了解我们针对客户研究提供的各种葡聚糖产品，建议您访问我们的碳水化合物分类页面。
如需了解生化试剂系列的更多信息，请填写此表。

可比产品

产品编号
说明
价格

FD500S

异硫氰酸荧光素-葡聚糖, average mol wt 500,000

FD40

异硫氰酸荧光素-葡聚糖, average mol wt 40,000

FD40S

异硫氰酸荧光素-葡聚糖, average mol wt 40,000

FD250S

异硫氰酸荧光素-葡聚糖, average mol wt 250,000

FD70S

异硫氰酸荧光素-葡聚糖, average mol wt 60,000-76,000

FD10S

异硫氰酸荧光素-葡聚糖, average mol wt 10,000

FD2000S

异硫氰酸荧光素-葡聚糖, average mol wt 2,000,000

FD20

异硫氰酸荧光素-葡聚糖, average mol wt 20,000

FD70

异硫氰酸荧光素-葡聚糖, average Mw 59,000-77,000

FD20S

异硫氰酸荧光素-葡聚糖, average mol wt 20,000

FD150S

异硫氰酸荧光素-葡聚糖, average mol wt 150,000

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H315,H319,H335

危险分类

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCM7525
BCCM2780
BCCK7322
BCCK8946
BCCK0284

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相关内容

Fluorescein Isothiocyanate-Dextran

Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the difference between FD4 and 46944?

    1 answer

    1. Products FD4 and 46944 are essentially the same product, but they are offered for sale by different divisions. The primary difference between the two lies in the amount of FITC conjugated to the dextran (glucose). For FD4, the amount of FITC ranges from 0.001 to 0.020, while for 46944, the range is 0.002 to 0.020. It is important to note that individual lots must fall within these specified minimum and maximum values.

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