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生物源
sugar beets root
共軛
FITC conjugate
形狀
powder
分子量
average mol wt 20,000
標籤範圍
0.003-0.020 mol FITC per mol glucose
顏色
yellow to dark yellow
溶解度
water: 25 mg/mL
儲存溫度
2-8°C
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一般說明
右旋糖酐是一种无水葡萄糖聚合物。由约 95% 的 α-D-(166) 键组成。其余 (163) 连接可解释右旋糖酐的分支。分支长度的冲突数据表明平均分支长度小于 3 个葡萄糖单位。然而，其他方法表明存在超过 50 个葡萄糖单位的分支。已发现天然右旋糖酐分子量 (MW) 在 900 万 ～5 亿范围内。较低分子量的右旋糖酐将表现出略少的分支，并且分子量分布范围更窄。分子量大于 10,000 的右旋糖酐表现得好像它们是高度支链。随着分子量的增加，葡聚糖分子的对称性增强。分子量为 2,000 至 10,000 的葡聚糖，表现出可膨胀弹簧圈的属性。在分子量低于 2000 时，右旋糖酐更呈棒状。采用以下一种或多种方法测定右旋糖酐的分子量：小角激光光散射法、分子排阻色谱法、铜络合-蒽酮试剂比色法测定还原糖含量和粘度。
應用
FITC-右旋糖酐作为荧光通量示踪化合物用于心血管、微循环、灌注、细胞单层和细胞膜渗透性研究，支持血流、膜损伤、血管引流和肾脏消除等过程的测量。小的 FITC-右旋糖酐也用于测量细胞内吞和细胞连接通透性等过程。荧光素-葡聚糖，20 kDa，可用于研究细胞过程，如内吞和渗透压。FITC-右旋糖酐-20 可用于研究内皮细胞或上皮细胞单层孔隙度、细胞层内顶端基底侧运动和细胞连接渗透性。FITC-右旋糖酐-20 可作为研究氢原子和胶原微棒等结构药物释放的模型化合物。
FITC-右旋糖酐利用显微荧光法广泛用于微循环和细胞渗透性研究。FITC-右旋糖酐已被用于研究植物细胞壁多孔性和毛细管渗透性。已证明血浆蛋白不与 FITC-右旋糖酐结合。
用异硫氰酸荧光素标记的葡聚糖可用于动物的灌注研究。
聯結
类似于 FD-20，但由 Sigma 生产。
準備報告
Sigma 通常检测浓度等于或高于 25 mg/mL 的 FITC 葡聚糖在水中的溶解度。溶液应避光保存。在体内，FITC-右旋糖酐可稳定 24 小时以上。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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相关内容
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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