D9281

Lambda DNA EcoR I Hind III Digest

for DNA electrophoresis

• UNSPSC代码: 41141827
• NACRES: NA.25

属性

• 质量水平: 100; 200
• 表单: liquid
• 用途: 100 uses (0.1 mg size); 500 uses (0.5 mg size)
• 适用性: suitable for electrophoresis (DNA)
• 储存温度: −20°C

说明

一般描述

Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

应用

Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

组分

10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA

其他说明

Contains 13 fragments, 125–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

制备说明

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

安全信息

• 象形图: GHS07
• 警示用语：: Warning
• 危险声明: H317
• 预防措施声明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危险分类: Skin Sens. 1
• 储存分类代码: 13 - Non Combustible Solids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable