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Merck

D6182

Sigma-Aldrich

DOTAP 氯化物

≥98% (TLC), powder

别名:

N- [1-(2,3-二油酰氧基)丙基] -N,N,N-三甲基氯化铵

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About This Item

经验公式(希尔记法):
C42H80ClNO4
分子量:
698.54
MDL编号:
UNSPSC代码:
12352211
PubChem化学物质编号:
NACRES:
NA.32
价格与库存信息目前不能提供

质量水平

方案

≥98% (TLC)

表单

powder

运输

dry ice

储存温度

−20°C

SMILES字符串

[H]C(C[N+](C)(C)C)(OC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O.[Cl-]

InChI

1S/C42H80NO4.ClH/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;/h20-23,40H,6-19,24-39H2,1-5H3;1H/q+1;/p-1/b22-20-,23-21-;

InChI key

KSXTUUUQYQYKCR-LQDDAWAPSA-M

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应用

1,2-二油氧基-3-(三甲铵)丙烷(DOTAP)氯化物已用于制备阳性脂质体和脂质体-DNA复合体。[1]它还用于评估肿瘤坏死因子α (TNF- α)的产生。[2]
由阳离子脂质体组成的化合物,用于向真核细胞中转染DNA、RNA及其他负电性分子。DNA与DOTAP混合时会自动形成稳定的复合物,后者可直接加入细胞培养基中。这些复合物能够与细胞膜融合并向细胞质中释放DNA。细胞被高效转染的同时不会产生细胞毒性。每μg DNA需使用5-10 μg左右的DOTAP,每mL 细胞培养基需加入约10-20 μg DOTAP。最佳的工作浓度和转染试剂视被转染的细胞系,以及转染试材类型(RNA、DNA、蛋白)而定。

生化/生理作用

1,2-二油氧基-3-(三甲铵)丙烷(DOTAP)氯化物可用于制备脂质体,且无需使用助磷脂。DOTAP氯化物可溶于二甲基亚砜(DMSO)和乙醇,借此可明确其作为转染试剂的作用。[3]

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

A novel strategy for tumour therapy combining cell apoptosis and active immunity induced by caspy2, a zebrafish caspase
Liu L, et al.
Journal of Cellular and Molecular Medicine, 13(8b), 2271-2281 (2009)
A simplified direct lipid mixing lipoplex preparation: Comparison of liposomal-, dimethylsulfoxide-, and ethanol-based methods
Meisel JW and Gokel GW
Scientific Reports, 6, 27662-27662 (2016)
Ju-mei Zhao et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22(12), 4272-4280 (2008-08-22)
The matrix (M) protein of vesicular stomatitis virus (VSV) plays a key role in inducing cell apoptosis during infection. To investigate whether M protein-mediated apoptosis could be used in cancer therapy, its cDNA was amplified and cloned into eukaryotic expression
Structurally distinct phosphatases CD45 and CD148 both regulate B cell and macrophage immunoreceptor signaling
Zhu JW, et al.
Immunity, 28(2), 183-196 (2008)

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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