See the link below to review the formulations of the DPBS products. These concentrations are reported in grams per liter:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/269/385/d5652for.pdf
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相关类别
一般描述
杜氏(Dulbecco′s)磷酸盐缓冲液(DPBS)是在组织培养中广泛使用的平衡盐溶液(BSS)。
应用
在制备和连续转移过程中，可使用D-PBS清洗细胞。
生化/生理作用
杜氏磷酸盐缓冲液可维持细胞渗透压平衡，作为灌洗、运输和稀释用液。它提供正常细胞代谢必需的水和大量无机离子。混入葡萄糖等糖后，这种缓冲盐可提供细胞代谢所需的主要能量。它的缓冲能力可使培养基维持在生理pH范围内。
数量
按照每升培养基9.6克粉末配制。
其他说明
该D-PBS配方中没有Ca或Mg。也可提供加入Ca和Mg的配方
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Zhaoxia Zhang et al.
Molecular vision, 24, 434-442 (2018-07-24)
Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression
Ana C Bohórquez et al.
Journal of colloid and interface science, 506, 393-402 (2017-07-28)
The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL
Functional enhancement of ultrafine Angelica gigas powder by spray-drying microencapsulation
Choi KO, et al.
LWT--Food Science and Technology, 101, 161-166 (2019)
Lauren E Prusinski Fernung et al.
The American journal of pathology, 188(10), 2293-2306 (2018-08-04)
Uterine fibroids (UFs) are clonal, hormonally regulated, benign smooth-muscle myometrial tumors that severely affect female reproductive health, although their unknown etiology limits effective care. UFs occur fourfold more commonly in African American women than in Caucasian women, and African American
Iqbal Hyder et al.
BMC molecular and cell biology, 21(1), 9-9 (2020-03-01)
Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity.
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What is the formulation for this buffer? Molarity of Na2HPO4, KH2PO4, NaCl, KCl, etc?
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Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?
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Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use. It can be autoclaved at 121°C, 15 psi for 20 minutes.
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?
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Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.
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What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?
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The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.
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What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?
1 answer-
This product contains 8.0 g/L sodium chloride.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?
1 answer-
Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.
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