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Merck

D0422

Sigma-Aldrich

DMEM - high glucose

With sodium bicarbonate, without ʟ-methionine, ʟ-cystine and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

DME, DMEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

杜氏改良 Eagle 培养基 - 高葡萄糖, With 4500 mg/L glucose and sodium bicarbonate, without L-methionine, L-cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

glucose: high
phenol red: yes
L-glutamine: no
sodium pyruvate: yes

運輸包裝

ambient

儲存溫度

2-8°C

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應用

Dulbecco′s Modified Eagle′s Medium(DMEM)是对Basal Medium Eagle (BME)的一种改良,含有四倍浓度的氨基酸和维生素。 原始的配方含有1000 mg/L的葡萄糖并用于培养小鼠胚胎细胞。 从此,它被按照多种方式进行修改以支持小鼠和鸡细胞的原代培养,以及多种正常和转化细胞。 这些培养基都可提供一种不同的L-谷氨酰胺和丙酮酸钠组成。 此外,葡萄糖浓度也被提高至4500 mg/L,因此被命名为“DMEM/High”。

重構

补充 0.584 g/L L -谷氨酰胺。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Ruth I C Glasgow et al.
Neurogenetics, 18(4), 227-235 (2017-10-28)
Mitochondrial diseases are characterised by clinical, molecular and functional heterogeneity, reflecting their bi-genomic control. The nuclear gene GFM2 encodes mtEFG2, a protein with an essential role during the termination stage of mitochondrial translation. We present here two unrelated patients harbouring
Stacey L Borrego et al.
Journal of lipid research, 62, 100056-100056 (2021-03-02)
Methionine (Met) is an essential amino acid and critical precursor to the cellular methyl donor S-adenosylmethionine. Unlike nontransformed cells, cancer cells have a unique metabolic requirement for Met and are unable to proliferate in growth media where Met is replaced
Yun Yeon Park et al.
Experimental & molecular medicine, 50(4), 46-46 (2018-04-28)
ATP depletion inhibits cell cycle progression, especially during the G1 phase and the G2 to M transition. However, the effect of ATP depletion on mitotic progression remains unclear. We observed that the reduction of ATP after prometaphase by simultaneous treatment
Hagar Elkafrawy et al.
Amino acids, 53(10), 1623-1634 (2021-09-15)
Plasma cysteine is associated with human obesity, but it is unknown whether this is mediated by reduced, disulfide (cystine and mixed-disulfides) or protein-bound (bCys) fractions. We investigated which cysteine fractions are associated with adiposity in vivo and if a relevant
John M Bekkers
Frontiers in synaptic neuroscience, 12, 18-18 (2020-05-20)
Neurons typically form daisy chains of synaptic connections with other neurons, but they can also form synapses with themselves. Although such self-synapses, or autapses, are comparatively rare in vivo, they are surprisingly common in dissociated neuronal cultures. At first glance

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