ATDC5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99072806, mouse unknown/unspecified, Epithelial-like

• 别名: ATDC-5 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: mouse unknown/unspecified
• 包装: tube of 5 μg 99072806-DNA-5UG; pkg of vial of cells 99072806-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse 129 teratocarcinoma AT805 derived

细胞系描述

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

应用

ATDC5 has been used to study cartilage differentiation.

培养基

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO₂; 37°C subculture every 2-3 days.

其他说明

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