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Merck

D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

DME/F-12, 1:1混合

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About This Item

MDL编号:
UNSPSC代码:
12352207
NACRES:
NA.75

产品名称

杜氏改良Eagle 培养基/营养混合物 F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

运输

ambient

储存温度

2-8°C

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一般描述

杜氏改良Eagle培养基(DMEM)是一种富含能量的常用细胞培养基,包含葡萄糖、维生素和氨基酸。杜氏改良Eagle培养基(DMEM)与Ham F-12的1:1营养混合物常用于细胞培养研究。为了弥补去除血清造成的缓冲能力损失,可以在配方中以15 mM的最终浓度添加4-(2-羟乙基)-1-哌嗪乙磺酸(HEPES)缓冲液。

应用

杜氏改良Eagle培养基/营养混合物F-12 Ham:
  • 已用作培养基的组分,以培养原代培养激活的人肝星状细胞
  • 已用于培养软骨切片
  • 可用于考德里氏体的繁殖

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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分析证书(COA)

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