JVM-13

96090516, human blood, Lymphoblastoid

• 分類程式碼代碼: 41106514

属性

• product name: JVM-13, 96090516
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: 46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian B-prolymphocytic leukaemia, EBV transformed

細胞系描述

Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.

應用

Study of B cell leukaemia

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18

培養基

RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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