L8

95102434, rat muscle, Myoblast

• UNSPSC代码: 41106514

属性

• 产品名称: L8, 95102434
• 生物来源: rat muscle
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Myoblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat Skeletal muscle myoblast

细胞系描述

The myogenic cell line L8 was isolated by D Yaffe in 1969 by selective serial passage of myoblasts from primary rat skeletal muscle cell cultures (new born, non-in-bred Wistar rats). Subsequently they were recloned by H Blau. L8 cells differentiate into cross-striated myotubes by fusion and express several muscle-specific proteins. The myoblast population will be depleted if cells are allowed to become confluent before sub-culturing. Therefore, sub-cultivation before confluency and periodical re-cloning is necessary. The preparation of a frozen stock at early passage is recommended.

应用

Study of muscle differentiation, expression and analysis of muscle-specific proteins

培养基

DMEM : Medium 199 (4:1) + 2mM Glutamine + 1% CEE (Chick Embryo extract (Seralab)) + 10% Horse Serum (HS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 cells to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C; sub-cultivate before confluency and reclone periodically.

其他说明

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