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product name
TM4, 88111401
生物源
mouse testis
增長模式
Adherent
染色體組型
Not specified
形態學
Not specified
產品
Transferrin, H-Y Ag, novel retinol-binding protein
受體
Androgen, oestrogen and progesterone receptors
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse BALB/c testis sertoli cell
細胞系描述
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
應用
Hormone studies
培養基
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
其他說明
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