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Key Documents

TM4

88111401, mouse testis, Not specified

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About This Item

分類程式碼代碼:
41106514

product name

TM4, 88111401

生物源

mouse testis

增長模式

Adherent

染色體組型

Not specified

形態學

Not specified

產品

Transferrin, H-Y Ag, novel retinol-binding protein

受體

Androgen, oestrogen and progesterone receptors

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse BALB/c testis sertoli cell

細胞系描述

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

應用

Hormone studies

培養基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

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