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生物来源
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髓瘤
Sp2/0 Ag-14
反应性
Anti-Measles Virus Nucleoprotein
技术
cell culture | mammalian: suitable
同位素/亚型
IgG2a
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse Sp2/0 x Mouse BALB/c spleen
细胞系描述
NP cl.25 monoclonal antibody was produced in vitro by fusing mouse myeloma cells with spleen cells derived from BALB/c mice immunised with purified measles virus. NP cl.25 is negative for haemagglutination and hemolysin inhibition as well as neutralisation assays and gives immunofluorescence only on fixed tissue. It has been used for measuring synthesis and topological accumulation of measles virus antigen during the lytic cycle.
免疫原
Measles virus (strain Hallè)
应用
Diagnosis, study of measles virus infection
培养基
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C
其他说明
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