MDBK Cell Line bovine

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90050801, bovine kidney, Epithelial-like

• 别名: MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• UNSPSC代码: 41106514
• eCl@ss: 32190102

属性

• 生物来源: bovine kidney
• 包装: tube of 5 μg 90050801-DNA-5UG; pkg of vial of cells 90050801-1VL
• 生长模式: Adherent
• 核型: Modal no. 51; hypodiploid
• 形态学: Epithelial-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Bovine kidney

细胞系描述

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

应用

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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