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Merck

BJ human cell line

94022524, IHW Number: 9254

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About This Item

分類程式碼代碼:
41106514

生物源

human blood

種族

Not specified

性別

male

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblastoid

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

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一般說明

Consanguineous: No
Homozygous: No

細胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA分析

A: 6901 3001
Cw: 1701 1203
B: 4201 3508
HLA DRB1: 15 11
HLA DQB1: 06 03

血清學資料

HLA A: 30 69
HLA B: 22 35
Bw4/bw6: 6

培養基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責聲明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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