CTLL-2

93042610, mouse blood, Lymphoblast

• 别名: CTLL-2 cell line
• 分類程式碼代碼: 41106514

属性

• product name: CTLL-2, 93042610
• 生物源: mouse blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C57b1/6 T cell

細胞系描述

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

應用

CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.

培養基

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml.

其他說明

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