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Key Documents

CESS

92110206, human blood, Lymphoblast

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About This Item

分類程式碼代碼:
41106514

product name

CESS, 92110206

生物源

human blood

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblast

產品

Surface Immunoglobulin ( Ig), MHC Class II

受體

Fc, complement

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human lymphoblast

細胞系描述

Derived from a patient with myelomonocytic leukaemia and subsequently exposed to Epstein Barr Virus (EBV). This line is EBV positive and therefore presumably derived from a normal B lymphocyte. This cell line is also positive for surface Ig, Fc receptor, complement receptor, surface Ia and EBNA. Responds to T cell replacement factor by increasing the number of IgG secreting cells. The cells should be handled under laboratory containment level 2.

應用

T cell replacement factor (TRF) assays, B cell differentiation studies

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,9
vWA: 16,17

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37°C.

其他說明

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