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生物源
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
骨髓瘤
P3X63Ag8.653
反應
Anti-IgM (polymorphic determinant), bovine
技術
cell culture | mammalian: suitable
同型
IgG2a
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
細胞系描述
The antibody is directed against a polymorphic determinant on bovine IgM. It reacts with serum IgM and IgM+ B cells, precipitating polypeptide chains of 80kDa and 28kDa. It does not react with bovine IgG.
免疫原
Bovine PBMs, depleted of T lymphocytes
應用
Evaluation of bovine immunoglobulin levels and lymphocyte populations
培養基
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells may require mouse macrophage feeder cells upon resuscitation.
其他說明
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