BCL1 Clone CW13.20-3B3

90061904, mouse blood, Lymphoblast

• UNSPSC代码: 41106514

属性

• 产品名称: BCL1 Clone CW13.20-3B3, 90061904
• 生物来源: mouse blood
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse BALB/c B cell leukaemia

细胞系描述

Derived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.

应用

B-cell differentiation studies

培养基

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates acc

其他说明

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