C127I

90060504, mouse breast, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: C127I, 90060504
• 生物来源: mouse breast
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse RIII mammary tumour

细胞系描述

Non-transformed clone from a RIII mouse. Supernatant fluid is negative for RT activity. Useful for visualisation of sarcoma virus-induced foci, in vitro quantitative assay for bovine papilloma virus. Suitable host for transformation with bovine papilloma virus DNA plasmids.

应用

Virus and transformation studies: Sarcoma virus, Bovine papilloma virus

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:15 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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