L132 (HeLa derivative)

89111004, human cervix, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: L132 (HeLa derivative), 89111004
• 生物来源: human cervix
• 生长模式: Adherent
• 核型: 2n = 46
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human cervix carcinoma

细胞系描述

Originally derived from a human embryonic lung. Used for primary isolation of enteroviruses and also found to be sensitive to hepatitis virus. The line shows HeLa markers. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

Isolation of enteroviruses

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. The cells grow in clumps.

其他说明

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