YTA 3.1.2

89040603, Reactivity Anti-L3T4 epitope B, mouse CD4 )

• 分類程式碼代碼: 41106514

属性

• 生物源: Rat x Rat Hybridoma
• ImmunoDon: DA rat spleen
• 骨髓瘤: Y3Ag1.2.3
• 反應: Anti-L3T4 epitope B, mouse CD4
• 技術: cell culture | mammalian: suitable
• 同型: IgG2b
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Not specified

細胞系描述

The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.

免疫原

CBA mouse spleen cells, thymocytes

應用

Not specified

培養基

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

例行更新培養

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37 °C. Saturation density 9 x 10⁵/ml

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.