KHOS-240S

86112810, human bone, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: KHOS-240S, 86112810
• 生物来源: human bone
• 生长模式: Adherent
• 核型: 2n = 46, aneuploid
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian osteosarcoma

细胞系描述

A revertant line of the KHOS/NP cell line (Sigma Catalogue number. 84102903). Established after overnight incubation at 40.5°C and then sub-cloned at 36°C. The line has similar properties to the parent HOS cell line. Cells are non-tumourigenic in nude mice and no longer possess a rescuable MSV genome.

应用

Tumourigenicity, transformation, biochemical and virus studies: MSV

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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