AC2

86061902, mouse blood, Lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: AC2, 86061902
• 生物源: mouse blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Interleukin 3 (IL-3)
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse lymphoblast

細胞系描述

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

應用

Growth factor studies, IL-2 assays

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

其他說明

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