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产品名称
TR6Bc1, 85042301
生物来源
mouse nerve
生长模式
Adherent
核型
Not specified
形态学
Glial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse C3H.He Schwannoma
细胞系描述
Ethyl nitrosourea induced tumour in C3H. The mice, originated in trigeminal nerve and brain; readily transplanted and resembles schwannoma histologically. Cultured cells form prominent processes, resemble 33B (Sigma Catalogue number. 85081901) morphologically, contain abundant C type particles.
应用
Tumourigenicity studies
培养基
DMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他说明
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