Vero C1008 [Vero 76, clone E6, Vero E6]

85020206, African green monkey kidney, Epithelial

• 别名: Vero 76 Clone E6 Cells, Vero Cells, Vero E6 Cells, VeroC1008 Cells
• UNSPSC代码: 41106514

属性

• 产品名称: Vero C1008 [Vero 76, clone E6, Vero E6], from African green monkey kidney, 85020206, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物来源: African green monkey kidney
• 生长模式: Adherent
• 核型: 2n = 60
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

一般描述

Vero cells are derived from the kidney of an African green monkey. These are anchorage-dependent cells that have applications in molecular and cell biology research.[1] Vero E6 cells enable achieving high titers of severe acute respiratory syndrome coronavirus (SARS-CoV virus)[2] and are also used in the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.[1]

细胞系来源

Monkey African Green kidney

细胞系描述

A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.

应用

VERO C1008 has been used for transfection, cultivation and propagation of West Nile virus[3], yellow fever virus[4], ZIKA[5], Ebola virus[6], Sabethes flavivirus (SbFV)[7], Crimean-Congo Haemorrhagic Fever (CCHF)[8], Human-derived Lake Victoria Marburg virus strain Popp.[9]

培养基

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他说明

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