45.6.TG1.7

90110506, mouse blood, Lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: 45.6.TG1.7, 90110506
• 生物源: mouse blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Immunoglobulin G (IgG2b) ( kappa)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c myeloma

細胞系描述

Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.

應用

Fusion partner, immunoglobulin production

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 4-5x100,000 cells/ml; 5% CO₂; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.

其他說明

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