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Merck

C7559

Sigma-Aldrich

氯仿

≥99%, PCR Reagent, contains amylenes as stabilizer

别名:

三氯甲烷

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About This Item

经验公式(希尔记法):
CHCl3
CAS号:
分子量:
119.38
Beilstein:
1731042
EC 号:
MDL编号:
UNSPSC代码:
12191502
PubChem化学物质编号:
NACRES:
NA.28
价格与库存信息目前不能提供

蒸汽密度

4.1 (vs air)

蒸汽压

160 mmHg ( 20 °C)

方案

≥99%

表单

liquid

包含

amylenes as stabilizer

包装

vial of 1.5 mL

技术

PCR: suitable

颜色

colorless

折射率

n20/D 1.445 (lit.)

沸点

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

密度

1.492 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, none detected

SMILES字符串

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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一般描述

氯仿在药物制剂中可用作麻醉剂和添加剂。现在它主要用作有机溶剂。[1]氯仿由人为和天然来源产生。这种在大气中含量丰富的卤化碳存在于地下水和地表水源中。[2]

应用

氯仿已用于苯酚-氯仿提取,以考察其从血液培养液中纯化核酸的能力。[3]它还用于 RNA 提取/分离[2]和纯化。[4]

象形图

Skull and crossbonesHealth hazard

警示用语:

Danger

危险分类

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

靶器官

Central nervous system, Liver,Kidney

储存分类代码

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

闪点(°F)

does not flash

闪点(°C)

does not flash

个人防护装备

Eyeshields, Faceshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Tissue engineering by self-assembly uses the cells' secretome as a regeneration template and biological factory of trophic factors. Despite the several advantages that have been witnessed in preclinical and clinical settings, the major obstacle for wide acceptance of this technology

实验方案

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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