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Merck

C3657

Sigma-Aldrich

Human Collagen Type V

from human placenta, powder, suitable for detection assays

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

product name

胶原蛋白 来源于人类胎盘, Bornstein and Traub Type V (Sigma Type IX), powder

生物源

human placenta

品質等級

形狀

powder

雜質

HIV, hepatitis B and hepatitis C, none detected

溶解度

aqueous acid: soluble

UniProt登錄號

應用

detection

儲存溫度

2-8°C

基因資訊

human ... COL5A1(1289)

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成分

所有胶原蛋白分子均由以三螺旋构象排列的三条肽链组成,其一级结构主要以三个氨基酸作为重复基序,即每隔两个氨基酸是一个甘氨酸残基,其前面的两个位置通常为脯氨酸或4-羟基脯氨酸。

注意

该产品应在-20℃下干燥保存,在此条件下可保持活性3年。

準備報告

该产物是通过Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984)的胃蛋白酶提取和盐分级分离法的改进制备的。它是一种酸溶性胶原,可以溶解在水中,并添加乙酸到pH值3.0(5 mg/mL),产生乳白色无色溶液。

分析報告

SDS聚丙烯酰胺凝胶电泳在和基底膜胶原蛋白相同的还原条件下测试,得到三条主要条带。

其他說明

胶原蛋白被分为许多种结构和遗传性不同的类型。 我们使用 Bornstein和Traub提出的系统命名法。 小心不要混淆Sigma命名系统和通用的胶原蛋白分类类型。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Gaoyan Tang et al.
Microbiology (Reading, England), 153(Pt 8), 2447-2457 (2007-07-31)
Adhesion of Aggregatibacter actinomycetemcomitans to extracellular matrix proteins is mediated by antennae-like surface structures composed of EmaA oligomers. EmaA is an outer-membrane protein orthologous to the autotransporter YadA, a virulence determinant of Yersinia. emaA was present in the 27 strains
The serotype a-EmaA adhesin of Aggregatibacter actinomycetemcomitans does not require O-PS synthesis for collagen binding activity.
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Identification of autoantigens and their potential post-translational modification in EGPA and severe eosinophilic asthma.
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The human oral pathobiont Aggregatibacter actinomycetemcomitans expresses multiple virulence factors, including the trimeric, extracellular matrix protein adhesin A (EmaA). The posttranslational modification of EmaA is proposed to be dependent on the sugars and enzymes associated with O-polysaccharide (O-PS) synthesis of
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Cyclic diguanylate (c-di-GMP) signalling affects several cellular processes in Bacillus cereus group bacteria including biofilm formation and motility, and CdgF was previously identified as a diguanylate cyclase promoting biofilm formation in B. thuringiensis. C-di-GMP can exert its function as a

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