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Merck

B4679

Sigma-Aldrich

BIS-TRIS丙烷

BioPerformance Certified, suitable for cell culture, ≥99.0%

别名:

1,3-双[三(羟甲基)甲氨基]丙烷

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About This Item

线性分子式:
CH2[CH2NHC(CH2OH)3]2
CAS号:
分子量:
282.33
Beilstein:
1786109
EC號碼:
MDL號碼:
分類程式碼代碼:
12161700
PubChem物質ID:
NACRES:
NA.25
价格与库存信息目前不能提供

等級

BioPerformance Certified

品質等級

化驗

≥99.0%

形狀

crystals

技術

cell culture | mammalian: suitable

雜質

endotoxin and bioburden, tested

有用的pH值範圍

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

應用

diagnostic assay manufacturing

異物活動

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES 字串

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI 密鑰

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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應用

  • 中和的嵌合DNA探针改善纳米线场效应晶体管中富含GC RNA的检测。: 在纳米线场效应晶体管的开发过程中,研究人员使用BIS-TRIS丙烷来检测富含GC RNA。该研究证实了缓冲液′在增进这些生物传感器特异性和灵敏度方面的作用 (Chou et al., 2019)。

  • 通过优化用于测定重组活化凝血因子VII药物产品中无γ-羧基谷氨酸(Gla)结构域杂质的阴离子交换色谱法来消除鬼峰(ghost peak): 在优化色谱法的过程中研究人员使用BIS-TRIS丙烷来消除药物杂质分析中的鬼峰。该缓冲液的′有效性确保了药物制剂中杂质准确可靠的检测(Tahmasbi et al., 2019)。

其他說明

很容易将 BIS-TRIS 丙烷产品规格与 BIS-TRIS 丙烷规格表进行比较

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Michael C Breadmore
Journal of chromatography. A, 1217(24), 3900-3906 (2010-05-11)
An EOF counter-balanced ITP boundary has been used to stack anions from high conductivity samples during continuous electrokinetic injection of the sample. In a polystyrenesulfonate/poly(diallyldimethylammonium chloride) polyelectrolyte coated capillary, the time at which the ITP boundary exited the capillary could
R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
E Csuhai et al.
Biochemistry, 34(38), 12411-12419 (1995-09-26)
A secreted dibasic cleaving peptidase capable of converting dynorphins into Leu-enkephalin-Arg6 was purified from the medium of EL-4 mouse thymoma cells. The enzyme is a novel metalloendopeptidase with a neutral pH optimum (6.9) and a molecular weight of approximately 130
S J Smith et al.
The Journal of biological chemistry, 259(15), 9365-9368 (1984-08-10)
The kinetics of binding and hydrolysis of ATP by bovine cardiac myosin subfragment 1 has been reinvestigated. More than 90% of the total fluorescence amplitude associated with ATP hydrolysis occurs with an apparent second-order rate constant of 8.1 X 10(5)

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